*Sadia Shakeel
Department of Oral Biology, University College of Dentistry, The University of Lahore, Pakistan

Ammara Chaudhry
Department of Oral Biology, University College of Dentistry, The University of Lahore, Pakistan

*Corresponding address: Department of Oral Biology, University College of Dentistry, The University of Lahore, Pakistan
Email: sadia.shakeel@ucd.uol.edu.pk

doi:
Keywords: Coronal; Dentin; Occlusion; Premolar; Radicular

ABSTRACT

Objective

This study aims to explore the histological alterations observed in the odontoblastic layer of the dental pulp following tooth preparation.

Methods

The research was conducted from May 2022 to May 2023 in Lahore, adhering to World Medical Association ethical guidelines. Participants aged 18-25 undergoing orthodontic extractions of healthy premolars were included, excluding those with dental issues. Teeth were divided into control and experimental groups, with the latter undergoing a 2mm preparation. Local anesthesia and high-speed handpieces with water spray were used for preparation. Teeth were extracted, fixed in formalin, processed, and stained for microscopic examination. Statistical analysis via SPSS 20 compared odontoblast layer changes in coronal and radicular pulp, with significance set at p < 0.05 and 90% study power.

Results

In this study, 40 individuals (23 males, 17 females) undergoing premolar extraction for orthodontics were divided into control and experimental groups. All 20 control samples showed normal odontoblast layers and nuclei in the coronal regions, while the experimental group had consistent abnormalities and absence of nuclei, with a significant p-value (<0.001). In the radicular area, the control group was normal, but the experimental group showed abnormalities in 11 samples, with nuclei absent in 8, yielding a non-significant p-value (≤0.100).

Conclusion

The findings shed light on the impact of injury on healthy dental pulp. Histological analysis following a 2mm coronal tooth preparation revealed normal radicular pulp, with histological alterations observed in the coronal pulp. These observations contribute to the existing literature on pulp responses to coronal tooth preparation.

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How to Cite this: Shakeel S, Chaudhry A. Histological Alterations in the Odontoblastic Layer of Dental Pulp After Tooth Preparation. J Sci Technol Educ Art Med. 2024;1(1):28-33

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